The NC Protein Extraction Kit, Nuclear & Cytoplasmic is desinged for rapid stepwise isolation of native, non-denatured cytoplasmic proteins and intact nuclei form cultured cells or tissue samples. The procedure, which is based on hypotonic lysis with mild detergents, is fast and simple.
The isolated proteins are ready for direct use in many proteomic or enzymatic activity applications, including immunoprecipitation, affinity purification and reporter gene assays. Cell extracts prepared using this kit are compatible with common downstream applications, including 1D and 2D electrophoresis,
Western blotting, electrophoretic mobility shift assays (EMSA) and MASS. Isolated proteins are also compatible with quantification assays such as bradford, lowry, and the BCA. The extracted nuclei can
also be stored frozen in the provided NE Extract Buffer. The intact nuclei further may be used for isolation of chromatin, histones, and nuclear RNA. Nuclear extracts are used in DNA-protein interaction assays (EMSA), 2D fractionation or other applications.