Search term PCR/RT-PCR Reagent has been found in the following products: 53

PCR/RT-PCR Reagent

M-MLV Reverse Transcriptase, M2410

  • Moloney Murine Leukemia RT
  • Absence of Contaminants
  • 200 units / ul
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Cat No Size Price
M2410-100 10,000 units $ 65.00 Add to cart
M2410-500 50,000 units $ 295.00 Add to cart
Unit Definition One unit is defined as the amount of enzyme required to catalyze the incorporation of 1nmol of deoxyribonucleotide into acid-insoluble forms in 10 minutes at 37oC, using poly(A)-oligo(dT)12-18 as the template-primer. Standard cDNA Synthesis Conditions50mM Tris-HCl (pH8.3), 75mM KCl, 3mM MgCl2, 10mM DTT, 1.0mM each dATP, dGTP, dCTP, and dTTP, 0.2 mg radom hexamer,1-5mg RNA, 200units M-MLV RT. The reaction volume was 20ml and the incubation was 45 min at 42 C.
Application First-strand cDNA synthesis.
Primer extension.
Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) is an RNA-dependent DNA polymerase that can be used in cDNA synthesis with long messenger RNA templates (>5kb). The enzyme is a product of the pol gene of M-MLV and consists of a single subunit with a molecular weight of 71kDa.