Search term Protein Electrophoresis Reagent has been found in the following products: 30

Protein Electrophoresis Reagent

Laemmli Sample Buffer(4X), Reducing , L1100

  • 4X Concentrated
  • Reducing
  • Trace Dye Included
Collapse Store at RT
Cat No Size Price
L1100-001 10 ml $ 20.00 Add to cart
Concentration(4X) Tris-Hcl(pH 6.8) : 250 mM
SDS : 8 %
Glycerol : 40 %
2-Mercaptoethanol : 8 %
Bromophenol blue : 0.02%
Application Protein Electrophoresis
Laemmli sample buffer is especially formulated for protein sample preparation to be used in the Laemmli SDS-PAGE system. A protein sample is mixed with the 4X sample buffer and heated in boiling water for 2-5 min. The 2-mercaptoethanol reduces the intra and inter-molecular disulfide bonds. The SDS detergent denatures the proteins and subunits and gives each an overall negative charge so that each will separate based on size. The bromophenol blue serves as a dye front that runs ahead of the proteins and also serves to make it easier to see the sample during loading. The glycerol increases the density of the sample so that it will layer in the sample well.