Reagents are optimized for greatest sensitivity of Firefly and Renilla luciferase signals
Very low background luminescence. No subtraction is required from readings.
Reliable, linear results for a concentration range over several orders of magnitude.
Compatible with Promega\'s Fluc and Rluc vectors.
Designed for assays with many different eukaryotic cell types (e.g. mammalian, lower invertebrates, etc.) using micro-plates or single-tube luminometers
The LucyQ Duo-Luciferase(Firefly & Renilla) Assay Kit provides an efficient means of performing two reporter assays. In the LucyQ Duo-Luciferase(Firefly & Renilla) Assay, the activities of firefly (Photinus pyralis) andRenilla(Renilla reniformisor sea pansy) luciferases are measured sequentially from a single sample.
The firefly luciferase reporter is measured first by adding FLuc working solution to generate a luminescent signal lasting at least one minute. After quantifying the firefly luminescence, this reaction is quenched, and the Renilla luciferase reaction is initiated simultaneously by adding RLuc working solution to the same sample. Both assays can be completed in about 4 seconds using a luminometer with reagent auto-injectors.