For optimal results and a long lasting glow kinetic please use following Gaussia-M2 sequence
(Welsh et al. , Multiply mutated Gaussia luciferases provide prolonged and intense bioluminescence,
Biochemical and Biophysical Research Communications 389 (2009) 563–568)
LucyQ Gaussia Luciferase Assay Kit contains reagents for measuring Gaussia luciferase activity in mammalian cell culture media and lysates. When used with Gaussia Vectors, the kit provides an extremely sensitive bioluminescent reporter assay system for secreted or intracellular detection of luciferase activity with extended signal stability. The light output generated by the luciferase reaction can be correlated to the amount of luciferase protein produced, which in turn is proportional to the activity of the promoter driving the luciferase expression. The kit can be used to detect the activity of any Gaussia luciferases or derivatives that use coelenterazine as a substrate.