Mycoplasma, represents a group of minute bacteria devoid of cell walls belonging to class Mollicutes. These are responsible for causing serious contamination in cell and or tissue cultures used to generate compendial articles. They may also cause contamination of filtered sterlized Soybean Casein Digest Broth.
Infection of cells in a culture can affect nearly every pathway of cell metabolism including alteration of the cells phenotypical characterization an dnormal growth. The presence of mycoplasma species does not always result in turbid growth in cultures or visible alteration of the cells. FRIIS Base Medium is a standard mycoplasma medium used for the cultivation of many mycoplasma excepti for Mycoplasma synoviae and cultivar a strains.