Search term Cloning Reagent has been found in the following products: 7

Cloning Reagent

fastLinky 5Min DNA LigationKit, 30 rxns, F0661

  • Ligation takes only 5 minutes.
  • Ligation is performed at +15°C to +25°C.
  • Simple procedure and Ready-to-Use
Collapse Store at -20℃
Cat No Size Price
F0661-030 30 rxns $ 91.00 Add to cart
F0661-150 5x30 rxns $ 335.00 Add to cart
Quality Control Each lot of the kit is pre-tested in a ligation reaction according to the standard protocol: 130ng of blunt-ended 2300bp insert DNA was ligated to 50ng of pUC19 DNA digested with SmaI and dephosphorylated with alkaline phosphatase. Yield of white colonies after transformation into competent E.coli XL1-Blue cells was >1x105 per μg DNA
Application The Rapid DNA Ligation Kit covalently joins either sticky-ended or blunt-ended DNA fragments
The fastlinky 5Min DNA ligation Kit enables fast sticky-end or blunt-end DNA ligation in only 5 min at RT. The kit contains T4 DNA Ligase and a specially formulated 5X FastLinky Ligation buffer optimized for fast and efficient DNA ligation. The efficiency of this fast ligation is analogous to that obtained with T4 DNA Ligase in a standard 1 hour ligation. The ligation reaction mixture can be used directly for bacterial transformation.