Each lot of the kit is pre-tested in a ligation reaction according to the standard protocol: 130ng of blunt-ended 2300bp insert DNA was ligated to 50ng of pUC19 DNA digested with SmaI and dephosphorylated with alkaline phosphatase. Yield of white colonies after transformation into competent E.coli XL1-Blue cells was >1x105 per μg DNA
The Rapid DNA Ligation Kit covalently joins either sticky-ended or blunt-ended DNA fragments
The fastlinky 5Min DNA ligation Kit enables fast sticky-end or blunt-end DNA ligation in only 5 min at RT. The kit contains T4 DNA Ligase and a specially formulated 5X FastLinky Ligation buffer optimized for fast and efficient DNA ligation. The efficiency of this fast ligation is analogous to that obtained with T4 DNA Ligase in a standard 1 hour ligation. The ligation reaction mixture can be used directly for bacterial transformation.