Search term DNA/RNA Purification Reagent has been found in the following products: 18

DNA/RNA Purification Reagent

DNase I(Pharmaceutical Grade), DN102

  • DNase I from Bovine Pancreas
  • Chromatographically Purified
  • >2,000 Kunits unit/mg
  • Lyophilized Powder
Collapse Store at -20℃
Cat No Size Price
DN102-001 100mg $ 510.00 Add to cart
Application Digestion of DNA in the recovery of intact RNA
Digestion of DNA in the recovery of intact Protein
DNA Mapping
Digest DNA from damaged cells
Removing membrane bound DNA fragments
DNase I is an endonuclease derived from bovine pancreas that will degrade double-stranded DNA in the presence of divalent cations, producing 3”-OH
oligonucleotides. Mg²⁺ in the reaction solution causes the enzyme to produce nicks in double-stranded DNA, while in the presence of Mn²⁺, DNase I cleaves both strands of the DNA. 

DNase I is useful in nick translation for introducing single-stranded nicks that serve as primer sites for initiation of DNA synthesis and for cloning random DNA fragments by cleaving double-stranded DNA. DNase I is a chromatographically pure preparation. It is offered as lyophilized powder. For greatest stability, it is important that DNAse be dissolved at a concentration of at least 1mg/ml in 50% Glycerol with 20mM Tris-HCl, pH 7.5 and 1mM MgCl2. This solution can be stored at -20oC for at least a year.