Search term Biochemical Reagent has been found in the following products: 29

Biochemical Reagent

DTT, 1,4-Dithiothreitol, D0505

  • CAS No : 3483-12-3
  • Formula : HSCH2CH(OH)CH(OH)CH2SH
  • MW : 154.25
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Cat No Size Price
D0505-001 1 g $ 32.00 Add to cart
D0505-005 5 g $ 55.00 Add to cart
D0505-010 10 g $ 97.00 Add to cart
D0505-050 50 g $ 336.00 Add to cart
D0505-100 100 g $ 483.00 Add to cart
Purity > 99%
UV Absorbance < 0.1 (280 nm , 1% solution)
An excellent reagent for maintaining SH groups in reduced state; quantitatively reduces disulfides. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol. Typically, a seven fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM).