Aprotinin inhibits the activity of several proteolytic enzymes such as chymotrypsin, kallikrein, plasmin and trypsin. Aprotinin is present in blood and in most tissues, with a high concentration in lung. Aprotinin inhibits pro-inflammatory cytokine release and maintains glycoprotein homeostasis. In platelets, aprotinin reduces glycoprotein loss (e.g., GpIb, GpIIb/IIIa), while in granulocytes it prevents the expression of pro-inflammatory adhesive glycoproteins (e.g., CD11b).
Aprotinin is a natural proteinase inhibitor polypeptide consisting of fifty-eight amino acids arranged in a single polypeptide chain, cross-linked by three disulfide bridges and having a molecular mass of 6512.
Source Bovine Lung
Physical Description Sterile Filtered White lyophilized (freeze-dried) powder
Formulation The protein (1mg/ml) was lyophilized with no additives
Solubility It is recommended to reconstitute the lyophilized Aprotinin in sterile water not less than 100ug/ml, which can then be further diluted to other aqueous solutions. .
Store at -20oC ; Lyophilized form stable at room temperature for 3 weeks, should be stored desiccated below -20oC .
Upon reconstitution, should be stored at 4oC between 2-7 days and for future use below -20oC.