Search term Cell Based Assay Kit has been found in the following products: 26

Cell Based Assay Kit

BrdU Cell Proliferation Assay Kit, C0300

  • Detects BrdU incorporated
  • ELISA Kit
Collapse Store at 2-8℃
Cat No Size Price
C0300-050 500 assays $ 587.00 Add to cart
C0300-100 1000 assays $ 886.00 Add to cart
C0300-500 5000 assays $ 3,085.00 Add to cart
Contents BrdU Reagent,
Fixing Solution
Prediluted Anti-BrdU Detector
Peroxidase Goat Anti-Mouse IgG
Conjugate Diluent
50X Plate Wash Concwentrate
Stop Solution
Application BrdU Cell Proliferation Assay

BrdU Proliferation Assay involves incorporation of BrdU into cells cultured in microtiter plates using the cell layer as the solid phase. During the final 2 to 24 hours of culture BrdU is added to wells of the microtiter plate. BrdU will be incorporated into the DNA of dividing cells. To enable antibody binding to the incorporated BrdU cells must be fixed, permeabilized and the DNA denatured. This is all done in one step by treatment with Fixing Solution. Detector anti-BrdU monoclonal antibody is pipetted into the wells and allowed to incubate for one hour, during which time it binds to any incorporated BrdU. Unbound antibody is washed away and horseradish peroxidase-conjugated goat anti-mouse antibody is added, which binds to the Detector Antibody.

The horseradish peroxidase catalyzes the conversion of the chromogenic substrate tetra-methylbenzidine (TMB) from a colorless solution to a blue solution (or yellow after the addition of stopping reagent), the intensity of which is proportional to the amount of incorporated BrdU in the cells. The colored reaction product is quantified using a spectrophotometer.