Search term Cell Based Assay Kit has been found in the following products: 26

Cell Based Assay Kit

CellCount-Blue Cell Proliferation Assay Kit, C0100

  • Non-Radioactive Assay
  • Sensitive
  • Homogeneous Single Reagent
  • Robust Assay
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Cat No Size Price
C0100-025 250 Assays $ 45.00 Add to cart
C0100-050 500 Assays $ 62.00 Add to cart
C0100-100 1000 Assays $ 102.00 Add to cart
C0100-500 5000 Assays $ 358.00 Add to cart
Application Cell Proliferation Test
Cytotoxicity Test
This homogeneous assay involves simply adding a single reagent, the CellCount -Blue reagent, to the cell culture and measuring the fluorescence intensity (excitation wavelength = 530-570 nm, emission wavelength = 590-620 nm after an incubation step. The CellCount-Blue reagent, utilizes the redox dye resazurin whichis not fluorescent, but upon reduction by metabolically active cells is converted into a highly fluorescent product (resorufin). Living cells can readily reduce this non-toxic reagent and the resulting increase in fluorescence intensity can be conveniently monitored using a luorescence specturophotometer or plate reader. Nonviable cells have no metabolic capacity and, thus, will not reduce the dye. Therefore, the fluorescence intensity observed in this assay is a true measure of the viable cells. The CellCount-Blue reagent has been optimized for maximum sensitivity, reproducibility and long shelf-life. The homogeneou cell-based assay can be performed in multi-well plates.

The reagent is comparible with all culture media and with all liquid handling systems for high-throughput screening applications in 96-well and 384 -well plates. Appli -cations include cell proliferation, cytotoxicity and apoptosis.