Search term PCR/RT-PCR Reagent has been found in the following products: 55

PCR/RT-PCR Reagent

AMV Reverse Transcriptase, 10u/μl, A1201

  • Avian Myeloblastosis Virus RT
  • Absence of Contaminants
  • 10 units / ul
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Cat No Size Price
A1201-200 200 units $ 122.00 Add to cart
A1201-500 500 units $ 239.00 Add to cart
Unit Definition 1 unit is the enzyme activity that incorporates 1 nmol of [3H]-dTMP into acid-precipitable products in 10 minutes at +37 °C with poly(A)+ · d(pT)15 as template primer.
Application First- and second-strand cDNA synthesis and synthesis of first strand cDNA
Dideoxy DNA sequencing
Primer extension
RNA sequencing
3′-end labeling of DNA fragments
AMV Reverse Transcriptase (AMV RT) catalyzes the polymerization of DNA using template DNA, RNA or RNA:DNA hybrides. It requires a primer (DNA primers are more efficient than RNA primers) as well as Mg²⁺ or Mn²⁺. The enzyme possesses an intrinsic RNase H activity. Both nonionic detergents and sulfhydryl compounds stabilize the enzyme activity in vitro.