Recombinant fusion protein containing a sequence corresponding to amino acids 1-451 of human alpha Tubulin
Purified polyclonal antibody supplied in PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
WB : 1:500 - 1:3000
ELISA : 1:5000 - 1:90,000
IHC : 1:500 - 1:2,000
Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Tubulin itself is a globular protein consisting of two poly-peptides (alpha and beta tubulin).
Alpha and beta tubulin dimers are assembled to 13 proto-filaments that form a microtubule of 22-nm diameter. Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton-associated carboxy-peptidase.
Dety -rosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation, which is characteristic of microtubules in neuronal cells and the mitotic spindle. This antibody makes an excellent loading control.