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Product Overview

Unique Hot Start and Fast Taq Activation in 20 Sec

amfiSure qGreen & qProbe Q-PCR Master Mixes contain heat-labile chemical blocker which enables standard Taq DNA Polymerase to be inactivated securely during early PCR cycle and to be activated in 20 seconds at 95°C.

Improved Reproducibility & Specificity

amfiSure Hot StartTaq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers.

Detect Low Copy and Difficult Targets Consistently

Improved processivity result in earlier Cq scores
Higher fluorescence detection across varying AT- and GC- rich targets

Complete real-time PCR in just 29 minutes, Fast PCR

60% shorter run times with fast cycling protocol
Maintain high performance when switching from standard to fast protocol

Non-Fluorescence Pinkish Mix For Visual Confirmation

amfiSure qGreen & qProbe Q-PCR Master Mixes contain non-fluorescence pinkish dye provides visual confirmation that not only has
the enzyme been added, but that proper mixing has occurred.

Less Viscosity for Less Pipetting Error

amfiSure qGreen & qProbe Master Mixes are less viscous than other competitors. It will provide easier pipetting and make less pipetting error.
 
Secure inactivation by heat-labile chemical based blocker
Faster activation within 20 sec compaired by Tag-antibody based
Less PCR inhibion campaired by Tag-antibody inhibition
 

Solid line, amfiSure qGreen PCR Master Mix
Dashed line, Competitor's SYBR Green Master Mix

GAPDH gene was amplified from MEF Cell line cDNA using amfiSure qGreen
Master Mix (1:10 to 1:2000 dilution). NTC = no template control.

Melting curve comparision for MEF cell line cDNA in real time, GAPDH endogeneous Control

GAPDH, Bmal 1 and Clock gene was amplified from total MEF cell line cDNA
using amfiSure qGreen Q-PCR Master mix or the competitor SYBR Green PCR
kit (Bio-Rad) cycling conditions: 95°C, 60s; 60°C

Product Information

Cat.No. Product Name Cat.No. Product Name
Q5600 amfiSure qGreen Q-PCR Master Mix(2X), Without Rox Q5601 amfiSure qGreen Q-PCR Master Mix(2x), Fluorescein
Q5602 amfiSure qGreen Q-PCR Master Mix(2X), High Rox Q5603 amfiSure qGreen Q-PCR Master Mix(2X), Low Rox
Q5700 amfiSure qProbe Q-PCR Master Mix(2X), Without Rox Q5701 amfiSure qProbe Q-PCR Master Mix(2X), Flurescein
Q5702 amfiSure qProbe Q-PCR Master Mix(2X), High Rox Q5703 amfiSure qProbe Q-PCR Master Mix(2X), Low Rox